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Hemin (0.8 mmol, 500 mg) was transferred in an inert atmosphere box.
For hyperoxic experiments, cells were incubated in the atmosphere box for 6 h at 40% O2 following addition of BTP.
For hypoxic experiments, cells were incubated in the atmosphere box for 6 h at 0.2% O2 following addition of BTP.
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Since OTS is easily hydrolyzed with the atmospheric moisture, this solution was always prepared under nitrogen atmosphere (glove box) and used immediately.
Goethite was dried under a nitrogen stream at 40°C and stored in a nitrogen atmosphere glove box.
For the experiments prepared in the absence of CO2, preparation, sampling, and reagent storage were done in a nitrogen atmosphere glove box.
Unless otherwise indicated, all materials and solvents were used as received, without further purification, and stored in an argon atmosphere glove box (O2, H2O ≤ 2 ppm).
The water was then transferred to a nitrogen atmosphere glove box and used to make FeCl3, SrCl2, KOH, and NaOH stock solutions from solids that were purged for 20 to 30 minutes under a nitrogen stream and weighed in the nitrogen atmosphere glove box.
Based on the results of transport tests, engineering scale process equipments connected each other with the transport technology have been developed, and installed in the Ar atmosphere glove box for integrated tests.
The substrates were cleaned using ultrasonic waves in a sequence of acetone, ethyl alcohol, deionized water, and ethyl alcohol, then dried using a stream of nitrogen gas and stored in a nitrogen atmosphere glove box.
Current-sensing atomic force microscopy (CS-AFM) was used to study the doping level distribution in electrochemically doped poly[2,2'-bithiophene] under a controlled atmosphere (glove box) to preserve the doping level during the measurements.
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