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To identify pathways that mediate SET8 recruitment to DSBs, we focused on proteins that influence 53BP1's recruitment to DSBs such as ATM 1 and HDACs 20, 30.
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The measured sensitivity is approximately 24 mV (V atm)−1, which is similar to that of traditional pressure sensors.
The deposition of the coating (approximately 7.0 μm thick) led to a dramatic decrease in the oxygen permeation across empty capsules at 40 °C and 25% RH (uncoated capsules: 496.91 mL [package 24 h atm]−1; coated capsules: 14.01 mL [package 24 h atm]−1).
In the presence of coffee inside the capsules, the barrier performance was even improved (pack permeability 1.87 mL [package 24 h atm]−1), which was ascribed to the residual oxygen inside the capsules at the beginning of the analysis.
Molybdenum and molybdenum-potassium catalysts supported on carbon and on alumina have been studied over a range of conditions, (1 atm, 1 1 & 2 1 H2/CO ratio) to investigate their usefulness as specific catalysts for C2 C4 hydrocarbons.
The experimental design was done at five levels and the studied ranges for temperature, pressure, solvent to pine barks ratio and tetralin to solvent mixture ratio were 300 400 °C, 10 50 atm, 1/1 5/1 and 0/1 1/1, respectively.
The blots were incubated with primary antibodies against p-ATM (1 1000, R&D Systems, MN, USA), ATM (1 3000; Gene Tex, San Diego, CA, USA), β-actin (1 3000, Beyotime Biotech).
The primary antibodies used in this analysis were rabbit monoclonal anti-phosphorylated ATM 1 1,000 (Abcam), mouse monoclonal anti-ATM 1 10,000 (sigma), rabbit monoclonal anti-phosphorylated CHK1 1 200 (Cell Signaling), mouse monoclonal anti-CHK1 1 500 (Abcam), mouse monoclonal anti-cyclin E 1 200 (Santa Cruz Biotechnology), and rabbit polyclonal anti-beta actin 1 5,000 (Abcam).
The oxygen concentration in the air-type sites was computed using Henry's law to be 260 μM (assuming the partial pressure of O 2 to be 0.2 atm and a Henry's law constant of 0.0013 mol l −1 atm −1 [ 14]).
The checkpoint pathway consists of several kinases, such as ataxia telangiectasia mutated protein (ATM [ 1, 2]), ataxia telangiectasia and Rad3-related protein (ATR [ 1, 2]), checkpoint kinase 1 (Chk1 [ 3, 4]), and checkpoint kinase 2 (Chk2 [ 5– 8]).
The lowest OTR level was 3.0 mL m-2 day-1 atm-1, which corresponds to an oxygen permeability of 0.04 mL mm m-2 day-1 atm-1.
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