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Although all parameters identified for the decision algorithms have been used as diagnostic markers of EOS earlier [ 25] this analysis allowed to identify cut-off values for AB treatment failure, as determined by the data, at well defined time points.
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5 × 103 cells were plated in 96-well plates, incubated for 24 hours at 37°C, and treated with specified agents at defined time points.
At the defined time points, animals were sacrificed, and the eyes were collected for histology.
Invasive sampling can be carried out under controlled conditions repeatedly and at accurately defined time points.
To measure the effect of sera on cellular growth, total protein was measured at defined time points during culture in 6-well plates by the colorimetric method [ 37].
Developmental stages (including developmental delays and failures) as well as mortality rate and hatching rate were observed at defined time points (Appendix 1, Table 5) using binoculars (Stemi 2000-C, Zeiss, Oberkochen, Germany).
Samples of supernatants were collected at defined time points and stored at −80°C until end point titration assays (TCID50) in MDCK cells.
At defined time points, 100 μl of the cell suspension in each well was quenched with 200 μl acetonitrile containing an internal standard.
Interestingly, reciprocal trends bend at similar age, suggesting that major expression changes are at defined time points.
Leaves harvested at defined time points were frozen with liquid nitrogen and then stored at −80°C.
The primary PK metrics was the average concentration of LNG in plasma at defined time points under stable conditions.
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