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LB-pH4: Tenfold diluted LB medium at pH4 was adjusted to 4.0 at 37°C and filter sterilized.
Pain at rest was adjusted for baseline score and age.
The volume of CO administered at altitude was adjusted according to barometric pressure.
The study consisted of 196 consecutive patients of which none received additional rehabilitation beyond an instructional exercise plan at discharge, which was adjusted at one in-patient visit.
The ion source voltage was set at 4800 V, declustering potential was adjusted at 30 V and focusing potential at 100 V.
Ethanol was removed under vacuum at 30 °C, and the volume was adjusted at 1 mL.
Cultivation was carried out at 30°C and the pH was adjusted at 7.0 using ammonium hydroxide.
The mixture was stirred over night at 4 °C and was adjusted to pH 7.8 the next day.
The pH was adjusted at 6.5 and autoclaved at 121 °C for 15 min.
The pH of the media was adjusted at 6.5 and temperature at 28 °C.
Initial pH of the medium was adjusted at 7.0 before sterilization at 121°C for 15 minutes.
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