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In crabs acclimated to 10-ppt salinity, free Ca2+ was maintained at twofold higher values than concentrations of the medium, and complexed calcium disappeared from the hemolymph after 10 days.
The results revealed that stable aqueous colloids and complete encapsulation of the lipophilic bioactive compounds into the micelles can be achieved at twofold excess of copolymer to propolis amount.
31 genes were expressed at twofold or greater in meiocytes than in seedlings and 49 genes were expressed at twofold or greater in anthers than in seedlings.
Gene expression analysis showed 40 differentially expressed genes at twofold change and P < 0.05.
In the present study, 40 differentially regulated genes were identified at twofold change and P < 0.05.
All samples were assayed in duplicate at twofold dilutions in assay buffer.
Similar(35)
The results showed that 26 miRNAs underwent at least twofold changes, significant at P < 0.05 (Table 1, Additional file 3).
Of the 45 sigma factors, expression of 18 was significantly and at least twofold enhanced between at least two consecutive time points.
mec-8; sqt-3 embryo keyhole depth was measured by growing embryos at 25°C starting at the twofold stage and quantified every 60 min for 5 hr.
We filtered out genes that did not change at least twofold (up or down) at any time point, and we used the default values for all other parameters.
Only genes, which exhibited at least twofold higher signal intensities in at least the first three time points at which samples were withdrawn, were considered here.
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