It is used at three steps of proteomic studies (Fig. 1): (i) identification of proteins by peptide mass mapping or peptide sequencing; (ii) prediction of their sub-cellular localization, (iii) prediction of their function.
Problems at one step of the manufacturing process can be compensated for in the next.
If an irrelevant propositum (e.g., 'The sun is behind a cloud.') is proposed at one step of a disputation and then again at a later step, a change in the weather may require a change in the appropriate response.
However, in our model, mixing is important at one step of the life cycle, after the growth phase, so the structure should not be maintained indefinitely (as pointed out by a reviewer).
These results suggest the presence of at least two steps of pore filling, one at very low P/P0 associated with the pores of molecular dimensions for effectively trapping the light into the inner layers and the other at a 0.7 1.0 of P/P0 associated with pores involving quasi-multilayer formation that proved the formation of PEI/TiO2 thin films which was our purpose [35].
After the end of the Last Glacial Maximum (LGM), Termination I recorded one of the fastest and highest amplitude eustatic oscillations of the Late Quaternary: in less then 15 kyr (between ca. 20 and 5.5 kyr cal. BP) sea level rose ~ 120 m, with at least two steps of increased rate of ice melting and eustatic rise, named Meltwater pulses 1A and 1B.
Let us consider Example 1 again, and suppose that someone tries to locate a point in V with fitness 1 in at most two steps of a certain (unknown to us) search algorithm.
Interestingly, the strongly up-regulated expression of Ib WRKY1 following HA suggested, in this instance, at least two steps of regulation were imposed: firstly, an early response of Ib WRKY, possibly under the control of S. littoralis-specific oral secretion that elicits SA burst.
As follows from thermodynamics energy for the H+-pump functioning is liberated only at the last two steps of water formation on addition of third and fourth electrons independently of the reaction pathway.
In the case of isothermal crystallization at Tc=290 K and higher, the two steps of the primary structure formation combine in a single isothermal step.
