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The phrase "at two separate times" is correct and usable in written English.
You can use it to indicate that something occurred on two different occasions rather than simultaneously.
Example: "The experiment was conducted at two separate times to ensure accuracy in the results."
Alternatives: "on two different occasions" or "at two distinct times".
Exact(19)
The showers are expected to peak over the East Coast at two separate times: in the early morning of Friday at 2 50 a.m. and early Saturday morning at 2 51 a.m.
It said that while the presenter appeared at two separate times in the ad, the totality of what she said in the ad was: "It's easy to play, if you do it online the Health Lottery checks it for you … and with over £100m handed out in prizes there are more winners too".
Lab practicals last for 35 minutes and are held at two separate times in two groups before the lecture exam.
The researchers also admit that data from these two separate species living at two separate times was pooled to provide results which were statistically significant.
DeJoria, 73, has a net worth of $3.1 billion, according to FORBES, but at two separate times in his life, he was homeless and living out of his car.
"For example, the methods of regression analysis were created at two separate times, one during the investigation of the shape of the earth and the motions of the comets, and the other during the investigation of inheritance and quantitative biology in the late 19th century.
Similar(41)
All patients underwent simultaneous blood flow measurements of both legs at two separate time points (5 26 hours) and medical data was collected from charts.
As stated previously, this outbreak arose at two separate time periods: 1) 1995–1999, reaching a peak in TB infections in 1996, and 2) late in 2001 2002, reaching a peak in TB infections in the summer of 2002.
This cluster arose at two separate time periods, with the first outbreak starting in the fall of 1995, peaking in 1996 and disappearing by the spring of 1999, with a second outbreak rapidly appearing in the fall of 2001, peaking in the summer of 2002, and disappearing in the spring of 2003.
As indicated earlier in this section, data collection took place at two separate time points.
In detail, effects of shape change have been found at two separate time windows.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com