Sentence examples for at the subsequent steps from inspiring English sources
To better understand the possible reasons for a reduction in the survival rate at the subsequent steps of cryopreservation, the concentration of amino acids, ammonium, γ-aminobutyric acid (GABA), soluble sugars, malondialdehyde (MDA), and oxygen were measured in garlic shoot tips undergoing cryopreservation.
At the subsequent step we subjected isolated histones to differential proteolysis by trypsin or Arg-C protease and analyzed peptides by LC/MS/MS with the use of alternating CID and ETD fragmentation of peptide ions.
Both Ca2+ and force spikes faded away as time progressed (15 to 20 mM K+), displayed higher frequency at the subsequent step (25 mM K+) and disappeared at holding potentials above 30 mM K+.
Homogenates were centrifuged at 4°C in the subsequent steps to remove nuclei, debris and mitochondria (Fig. 6A).
This was carried out overnight at 27°C, and the subsequent steps of the IHC protocol were performed by a commercial immunoperoxidase technique (Vector-elite ABC kit; Vector Laboratories, Peterborough, UK) and finally counterstained with Mayer's haematoxylin.
For the same reason, we did not systematically ask parents declining at each of the subsequent steps, especially pre-test and training participation, for their specific reasons for declining.
In addition, once searching has identified potentially relevant full-text articles (ie, at the 'end' of step 2), the subsequent steps often take place at approximately the same time.
For the remaining 62 preschools, the screening procedure focused on children aged 3 to 6 years who were expected to stay in preschool for at least one year and, therefore, would be applicable for the subsequent steps of the project at least up to the post-test immediately after the training.
DNase I treatment was conducted at 37 °C for 30 min, and the subsequent steps were conducted as recommended by the manufacturer.
All of the subsequent steps were performed at 4°C.
All the subsequent steps were performed at 4°C.
