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Current-source density analysis revealed the main source at the stratum pyramidale (SP) and a strongly attenuated source at the SLM, a pattern typical for theta222 (Fig. 2c).
Microinjection of the Ih blocker ZD7288 (0.1 mM, 2 × 50 nL) at the stratum lacunosum moleculare (SLM) in urethane-anesthetized animals decreased the power of OLMα2 cell-induced theta (Supplementary Fig. 4e, f).
Concurrently, there was diminished β-glucocerebrosidase activity at the stratum granulosum-stratum corneum junction and a modest decrease in both involucrin and loricrin protein expression, markers of keratinocyte differentiation.
Following transfer from a humid to dry environment, electron microscopy revealed a marked decrease in: (i) lamellar bodies in the outermost stratum granulosum; (ii) deposition of lamellar body contents at the stratum granulosum stratum corneum interface; and (iii) the quantity of intercellular lamellae in the stratum corneum, which together could account for the barrier abnormality.
From the initials 49.30 μg applied to the skin, 9.50 μg were quantified in the receptor medium, 20.48 μg was retained at the stratum corneum (do not account as permeated) and 21.41 μg was retained at the viable epidermis + dermis (account as permeated), totalizing 30.90 μg of resveratrol permeated after 24 h of application (62.6%).
This is primarily explored from the perspective of transitivity at the stratum of lexicogrammar.
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