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Undifferentiated cells of the same animals at the same passage were used as a reference.
Primary cells were used between passages 3 and 14, and experiments were performed using fibroblast cultures at the same passage.
Our results show that pGFs can be reprogrammed to pluripotency at least 7-fold more efficiently than TTFs at the same passage number derived from the same mouse.
Each set of experiments was done using cells at the same passage (ranging from 7 to 10), carefully matching AD and non-AD samples.
As expected, the level of reactive oxygen species (ROS) was significantly higher in Zfp277−/− MEFs than the wild-type MEFs at the same passage (Figure S3), suggesting that the loss of Zfp277 leads to enhanced oxidative stress which could promote senescence.
All differentiation assays have been done at the same passage.
Similar(41)
This difference was not statistically significant due to variation in TRF lengths in different samples of TERA2 even at approximately the same passage, suggesting that the TERA2 is not homogenous and likely contains a subpopulation of cells that are continuing to elongate telomeres and may be more rapidly proliferating.
About the funny sentence by Benchley — what I said was (and I think this is actually an important point) that it's funny in the paperback but not on the Kindle, because humor is sensitive to things like grayness and typographical infelicity — and then I say, at the end, that the same passage by Benchley is funny again on the iPod.
Midway through the book, he quotes Dalton in Spanish; at the end, he translates the same passage into English: "My country, you don't exist / You're only a bad silhouette of mine / a word I believed from the enemy".
There were four replicates at each time point grown in separate plates compared with normoxic cells from the same passage harvested at the same time.
ZH501 virus stock at CDC [16] and our ZH501 stock underwent the same passage history.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com