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The autofluorescence intensity from the surrounding bone was measured at the same emission and excitation wavelength as the insertion site.
For comparison, we also reconstructed the fluorophore distribution using CW data at the same emission wavelength.
Confounding effects of native fluorescence can be examined with negative controls at the same emission (green) and excitation wavelengths (blue) and otherwise identical experimental conditions.
Considering the pollutant concentrations at the same emission rates, the average ratio of 1,3-butadiene to benzene across all the different receptor locations in the modeling domain is 0.43, versus 0.92 for the average ratio of BaP to benzene.
As aggregates are only likely to be found in energized mitochondria, 575 630 nm images taken with either 405- or 561-nm excitation provide better discrimination than do images at the same emission wavelength made using 488-nm excitation, which produces spillover from extramitochondrial JC-1 monomers.
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Note that clumps located at different distances can produce the same emission at a specific wavelength.
In the case of ethanol, the nanoparticles show almost the same emission at approximately 404 nm as in hexane, but the plateau becomes nearly indiscernible with the termination at approximately 479 nm and a weaker emission at approximately 578 nm.
In 1,4-dioxane, 3 has excitation maxima at 233 nm and 363 nm, with both excitation wavelengths resulting in the same emission maximum at 469 nm, see Fig. 1d and Fig. S2 (ESI †).
All the nanostructures show the same emission maximum at 508 nm regardless of their morphology and dimension.
The blue-luminescent Au (blue curve) and blue-luminescent Pt (green curve) show nearly the same emission maximum at approximately 350 nm.
At the same time, emission intensity of the host ZnS nanocrystals decreased.
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