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Across all genotypes, water conductance was less at the base of the crown root than at the other sampling positions.
It can be inferred from Fig. 2 (a) that pH exhibit a significant difference among the sampling sites, where pH at outfall and downstream fell under the permissible limit (6.5 7.5) while at the other sampling sites exceeded the guidelines.
As expected, root hairs near the root tip were not fully developed, and both density and length were lower at the 5 cm position than at the other sampling positions.
The relatively high dissolved oxygen levels at sampling sites 1, 2 and 3 could be attributed to the influence of wave action enriching sea water with oxygen unto these sites, whilst the low dissolved oxygen levels recorded at the other sampling sites were due to pollution from agricultural and domestic sewage (Biney 1984).
Richness showed no variation at the other sampling times, except on day 56.
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The flexible-protocol and liberal re-sort strategies proved highly efficient at capturing 'rare' clusters, here defined as appearing in only one or two of the five sampling strategies, but were also equally successful as the other sampling strategies at capturing common clusters, here defined as clusters appearing in three to five sampling strategies (Table 1).
For sample A1, paired-end adapters (Paired End DNA Sample Preparation Kit, FC-102-1001, Illumina, San Diego, USA) was used at the same molar ratio as the other samples.
This matches the 10 puff data collection at the other sample points.
However, in this study, the Microbiota was not affected by high aluminium levels in the soil based on the similarity of this population to those at the other sampled sites.
Magnesium, calcium and silica nitrates and phosphates contents in water were significantly higher at site S1 as compared to the other sampling site showing a decreasing trend with the distance from site S1 (Table 2).
The six prevalent bacterial T-RFs (T-RFs 33, 83, 91, 147, 166 and 373 bp), with high relative abundance at P1, were also detected for the other sampling points (Fig. 4).
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