The phrase "at the highest conservation" is not correct and may be confusing in written English.
It could be used in contexts discussing environmental protection or resource management, but it needs clarification or rephrasing for better understanding.
Example: "The project aims to preserve biodiversity at the highest conservation levels."
Alternatives: "at the utmost conservation" or "at maximum conservation".
We also looked at the high conservation of at least one residue in each transmembrane helical segment (see above).
In contrast to the high conservation at C-terminal DNA-binding POU domains, N-terminal regions are very diverse among Brn-2, Brn-1 and Oct-1 (Supplementary Material, Fig. S5).
Bioinformatic prediction tools provide substantial evidence to functional consequences and the fact that we have not found a second heterozygous or homozygous c.899G > C carrier in a set of 1046 healthy subjects and the high conservation at this locus furthermore support a potential association with the phenotype.
Nevertheless, the high conservation score at FANCA rs61753269 (SiPhy =12.742), combined with its extreme rarity in the population (MAF <0.001 in 1000 Genomes and ESP), suggest that this variant is under strong functional constraint and therefore could have a specific role on protein conformation.
MEST parent-of-origin expression in M. domestica remains undetermined since no informative polymorphisms in parent-offspring duos were found; however, due to the high conservation of imprinting expression at this locus, it is likely that the gene is paternally expressed, as it is in another metatherian, the tammar wallaby [ 8].
Correspondingly, 17.3% and 12.8% of them were removed in the shuffling step, indicating that the filtering step is too conservative at the highest levels of sequence conservation.
As previously determined for other species [26], but at a much larger evolutionary scale, these comparisons reveal very little conservation at the highest level (i.e., for each E. bieneusi fragment the genes are found on many E. cuniculi chromosomes), but there were many blocks of genes where local gene order is highly conserved.
Relatedly, the position of the phylotypic stage varies between these two groups of genes: developmental gene expression shows highest conservation at mid-developmental stage, in agreement with the hourglass model while the conservation of housekeeping genes keeps increasing with developmental time.
There is high amino-acid sequence identity among the AA10 proteins whose structures have been determined, with the highest sequence conservation observed at the active site (magenta color).
This supports the hypothesis that the sharp decrease of recessive disease genes at high conservation levels could be related to the paucity of human essential genes in the recessive disease set.
