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The final amplicon was obtained at the expected length of 372 nt after 35 cycles.
One-step protocol was then followed in a reaction mixture of 50 μl according to the manufacturer's instructions and final amplicons were obtained at the expected length after 35 cycles.
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So, the repair of AP sites initiated by Tdp1 fully restored the intact DNA and generated the products of the expected lengths at each intermediate stage.
To investigate the possibility that FGF9 can act in an autocrine manner through FGF receptors on HET-1A cells, RT-PCR of isolated mRNA identified FGF receptor isoforms 2 and 3. A transcript for both FGFR2 and 3 was detected at the expected lengths of 154 bp and 150 bp, respectively (fig 1C, n = 3).
Static pull tests on the new bolt show that it can elongate to any expected length at a high load level, thereby absorbing a large amount of energy to maintain the stability of surrounding rock.
Of the 14,618 mouse genes annotated through the union of all libraries, almost one half (7,060) had at least one putative CDS reconstructed at > 99%, 9,290 at > 90%, and 10,104 at > 80%, of the total expected length.
Given that, we reconstructed 8,325 distinct mouse isoforms out of 24,338 (~34%) for at least 90% of the total expected length, we established an important set of sequences, likely orthologous to mouse genes, which will be useful for future analyses of molecular evolution, population genomics, and phylogenetics.
Patient 5 (Table 1, Figure 2C) was one of the patients who seemed not to respond to THC, at least regarding expected length of survival.
At each site, the director was asked to provide a list of 20 patients who had expressed interest in taking part in the study, all of whom had an expected length of stay of at least 3 months.
Expected length of the procedure was approximately 1 h.
We studied 138 patients admitted to the general intensive care unit (ICU) at the Karolinska University Hospital, Solna, Sweden, with creatinine clearance >60 mL/min/1.73 m, estimated by the modification of diet in renal disease (MDRD) equation, and an expected length of stay of at least 24 hours.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com