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When we compared undifferentiated (proliferating) and differentiated (non-proliferating) murine and human podocytes, we found expression of cofilin-1 and ADF was only slightly increased in both species at the differentiated state.
Based on the assumption that cells at the self renewal state are represented by a stable limit cycle, we present a detailed, up-to-date mathematical description of the Delta1/Notch1 signalling pathway and demonstrate the behaviour of the system not only during the oscillatory phase but also during the transition towards specific cell lineages and at the differentiated state.
Again the interpretation in terms of three folding domains is clear when looking at the differentiated data in the Supporting Information.
Low levels of Oncopeltus zen expression are found along the entire circumference of early blastoderm embryos and resolves into a complex pattern that spans the egg circumference at the differentiated blastoderm stage (Panfilio et al., 2006).
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This expression pattern is also observed at in the differentiated myotome at E11.5 (Figure 1B).
At this size, the differentiated temperature treatment was ended.
The data indicated that this aptamer bound efficiently to mESCs, but failed or only weakly bound, if at all, to the differentiated mouse cell lines (Iwagawa et al. 2011).
However, that did not occur at the expense of the differentiated populations since the fraction of Delta+ cells remained unchanged and cell differentiation appeared to be unaffected (data not shown).
At each experimental endpoint, the differentiated cells were identified by immunostaining using the Tuj-1 and GFAP antibodies shown in Supplementary information, Table S2, or total RNA was extracted for RT-PCR analysis.
Indeed, HMGA1 is located in the cytoplasm in anaplastic thyroid carcinomas, colon and breast carcinomas, whereas it is located only at nuclear level in the differentiated thyroid carcinomas, in situ breast carcinomas and in colon adenomas.
In addition, the differentiated cells at the regenerating region undergo de-differentiation to form epithelial stem cells, which then re-differentiate to complete the regeneration process [ 41].
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