The in vivo 2D L-COSY technology was historically developed by studying the posterior cingulate gyrus (PCG); as at that time in the development of the technology, it generated the strongest signal due to proximity to the coil.
These results are likely to be of biological significance, as our data and other published results indicate that (1) EphB2 is expressed in cortex at this time in development [20], [27], (2) EphB2 is found at synapses [13], and (3) loss of EphBs from cortex results in abnormalities in synapse and spine formation [16], [22].
Analysis of multiple embryos also shows that Xist and Oct4 expression levels are not correlated at the 8-cell stage, although transcription of both genes is up-regulated at this time in development.
On top of this it is packed with interesting information, both about Britain at that time in history and about the development of computers, which I found fascinating.
Analytic methods, such as inspection or model building can take place at any time in development, but may not reflect the issues that emerge during actual use.
Genes that maintained their differential expression across all time points, likely reflect region-specific differences between cortical and spinal cord- derived progenitors while genes whose differential expression is not maintained may reflect a biological process that is going on at a particular time in development.
We reasoned that if Abd-A repressed the expression of the neuropeptide it would do so at any time in development, whereas a change of neuronal fate would be likely to occur only in early development.
The expression level of each SSTF node is entered for each cell type, at each time in development.
The more C4 activity an animal had, the more connections between cells were eliminated at a key time in development.
However if the system is non-linear and the fluctuations occur at an early time in development, where levels are still low, fluctuations may lead to different patterns.
Interestingly, our mannose experiments revealed a small window of optimal mannose supplementation at the earliest time in development: mannose was only effective if provided prior to 24 hpf.
