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At suitable time points, the optical density of the wells was determined using an ELISA plate reader (Molecular Devices, Sunnydale, CA, USA) set at 450 650 nm.
SPAD measurements were taken at several time-points after anthesis, aiming for an initial reading when there was no visible difference between the NILs, and then at suitable time points thereafter as senescence progressed.
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One measurement is made in wild type cells and the other measurement in the mutated strains at a suitable time point.
Therefore, week 24 may be a well suitable time point to measure long term responses.
In order to determine the most suitable time point for detection, we performed adipogenic induction for 30 days.
Therefore, we determined that 30 DAP is a suitable time point for seed transcriptomic analyses.
The expression of salt-responsive genes was analyzed for each sample in order to determine the suitable time point for samples to be sent for sequencing (Additional file 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2014/467395).org/10.1155/2014/467395
Therefore, we agreed that performing the primary outcome assessment after the end of treatment is a relatively suitable time point.
Infected tissues were examined under a microscope at 12, 24, 48 and 96 hpi, to determine the most suitable time-points for microarray analysis and to observe sporulation.
The sleep time allows enforcing Iptables' rules at suitable times.
The NCBI GEO database contains nine datasets that studied C. elegans gene expression under different conditions or at different time points and therefore are suitable for expression coherence analysis.
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