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Cells were harvested at stated time points.
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At the stated time points, fatty acid esters were extracted and quantified as follows.
Fetal and placental wet weights were taken at the stated time points after a discernible plug.
Embryonic and placental wet weights were taken at the stated time points after a discernible plug.
Owing to the adaptive characteristic of seizures in Syn KO mice (Etholm et al. 2012), only one provocation for each animal was performed at the stated time points.
Gentamicin was added after an additional 30 min. Ten millimolar alkDala or azDala were then added at the stated time points.
When time course experiments were performed, the mice were prelabelled with 12.5 μCi Ca and the kinetics of the release of Ca was analysed by withdrawal of small amounts of medium at the stated time points.
To assess the degree of asexual sporulation exhibited by Z. tritici strains at the stated time points post inoculation, leaf samples were kept for a further 48 h at 100% relative humidity in darkness at 18 °C.
Neither of these publications detailed cough type (wet or dry), the predictors of cough persistence or the outcomes (i.e. diagnosis or treatment) beyond the stated time points.
Pregnant females were sacrificed at stated embryonic time points.
This practice developed into set common prayer at stated times each day (matins or vigils, midnight; lauds, first daylight; prime, sunrise; terce, mid-morning; sext, noon; none, mid-afternoon; vespers, sunset; compline, before retiring).
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