at specimen-level pixel sizes of 0.223 nm or 0.29 nm, respectively, using a dose of ~30 e− Å−2 and a nominal defocus range of −1 to −3 μm.
Statements of DSC and validation at the specimen level and at the boundary value problem levels are also presented.
Data were acquired using a Tecnai F20 Twin transmission electron microscope at 120 keV at a nominal magnification of 80,000× (1.45 Å at the specimen level) using low-dose exposures (∼20 e−Å−2) with a randomly set defocus ranging from −0.5 to −1.3 μm.
The CDEs include cancer registry data (demographic, epidemiology and follow-up) at the patient level, pathology data at the specimen level including data elements to elaborate TNM staging and tumor grading, along with block level annotation and genotype data [ 16].
Images were collected at a nominal magnification of 49,000 (2.18 Å/pixel at the specimen level) with an electron dose of 20 30e−/Å using a 4K × 4K TVIPS TemCam-F416 CCD camera and the Leginon software package (Suloway et al., 2005).
Wild-type particles were visualized on a 120 kV TEM (FEI), and images were recorded at a nominal magnification of 52,000 × at the specimen level on a 4 k × 4 k CCD (Gatan).
The mutant complexes and gold-labeled particles were visualized on a 120 kV TEM (FEI) and recorded at a nominal magnification of 68,000 × at the specimen level on a 4 k × 4 k CMOS-based camera (Tietz).
Micrographs were digitized on a SCAI microdensitometer (Zeiss) at a sampling rate of 1.4 Å per pixel at the specimen level.
Data were collected using a Philips CM-100 microscope fitted with a 1K×1K Gatan Multiscan 794 CCD camera with magnification set at ×61000, which corresponds to 3.93 Å per pixel at the specimen level; the underfocus value was adjusted to ∼1.0 μm.
For the initial 3D random conical tilt reconstruction, tilt-pair images were recorded at 0° and 50° at 30,000 nominal magnifications with the same setup (3.56 Å/pixel at the specimen level).
