Sampling of the supernatant liquid was realized at specified time intervals.
At specified time intervals (0, 30, 60, 120, 180, 240, and 480 min), supernatants were collected by centrifugation.
The samples were collected at specified time intervals, and the concentration of CV in the effluent was analyzed using UV/vis spectrophotometer.
At specified time intervals of 5 min, films were removed from PBS solution and excess PBS was removed with filter paper until the films degraded.
Effluent samples from the column were collected at specified time intervals, and remaining lead concentrations in the solution were measured by atomic absorption technique.
At specified time intervals, an aliquot (5 ml) of the dissolution medium was removed and the concentration of CP was determined by UV absorption measurements, respectively, at 205 and 217 nm for the acidic and basic buffers.
At specified time intervals, 380 μL of the samples were transferred to a 2 mL LC amber vial, and fortified with sucralose- d6 internal standard (20 μL of 10 μg/mL) to a final volume of 400 μL.
The function of this module is to find the optimal configuration for a particular set of valves at specified time intervals during simulation period, which results in the highest NPV value.
At specified time intervals (12, 24, 36, 48, 60, and 72 h), the culture dish was taken out of the reactor and the Av concentration of samples was analyzed.
Blood samples (0.2 ml) at specified time intervals i.e. 0.5, 1, 2, 3, 4, 6, 8, 12, 24, and 48 h were withdrawn from retro orbital choroid plexus (under mild anesthesia), collected in EDTA-tubes and finally the plasma was separated through centrifugation (rpm = 400, time = 10 min) and stored (− 40 °C), until further analysis through UPLC-ESI-Q-TOF MS/MS UPLC-ESI-Q-TOF MS/MSin-hoUPLC-ESI-Q-TOF MS/MS
