Specifically, the temperature is assumed to be 25° C with reagents at specified concentrations.
A more specific physical interpretation of the +1.10 volt value is that it represents the voltage that would be produced by an ideal electrochemical cell based on the copper II) ion to copper metal and zinc II) ion to zinc metal half reactions with all the reagents at specified concentrations.
Yeast Cof1 or mouse cofilin1 was incubated at specified concentrations with polymerized actin for 20 min at room temperature.
Unless specified in the results and figure legends section, an assay mixture contained 40 nM template-primer, ∼20 nM of active WT or mutant D211N Ty1 RT, with PPi donor and MgCl2 or MnCl2 at specified concentrations.
For experiments involving inhibitors, explants were first cultured in imaging media (pre), which was then replaced by imaging media containing the inhibitor at specified concentrations for 30 minutes (during).
Nucleosomal arrays or histone-free DNA (200 ng), created using SfaNI digested PRST5, were incubated for 15 min at room temperature, in the presence of TRF2 or truncated mutants at specified concentrations, with 100 mM NaCl and reaction buffer containing 50 mM HEPES, 1 mM DTT and 2% glycerol.
Then EGCG or DMSO (vehicle control) was added after plating onto adherent cells at specified concentrations.
For experiments with rapamycin (Selleckchem) exposure, cells were incubated with rapamycin at specified concentrations for 12 hr posttransfection.
SnOCl2 or SnOCl2·pyridine was prepared at concentration of 5 µg/ml in different buffers adjusted with citrate for lower pH or supplemented with imidazole at specified concentrations.
F127 solution was added at specified concentrations to ∼30 μL of CVM at a 3% (v/v) ratio in custom-made chamber slides.
K562 cells were plated into 6 well plates at 3 × 10 cells per well overnight, then treated with PPD at specified concentrations for 48 h.
