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However, construction of 3D-tissues at single cell level has yet to be reported.
Until very recently, it was not possible to quantify the uptake of a given compound at single cell level.
The concept is demonstrated at single cell level using both H adsorption and CO stripping, and the H adsorption (cathodic current) method is extended to stack testing.
Although quorum sensing-dependent regulation is a major factor activating galactoglucan production, activities of both promoters did not correlate at single cell level.
We demonstrate an integration method of protein patterns in microfluidic chambers which allow cell attachment at single cell level and long term cell culture.
Finally, experimental data at single cell level obtained using hybrid cells suggest that the proposed hypothesis applies only to the irradiated component of the hybrid.
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Here we present the validation of a microfluidic-chip-based method for detecting EGFR multi-mutations at single-cell level.
For cell samples fewer than 105 cells, the emerging microfabrication technologies have advanced the examinations of protein expression or gene mutation at single-cell level by preciously controlling single cells and their surrounding environments.
To address this requirement, we developed a simple microfluidic chip to simultaneously finish on-chip cell identification and in situ cell lysis for detecting EGFR multi-mutations at single-cell level.
Therefore, it offers a simple and reliable method for detecting telomerase activity at single-cell level without complex pre-modification of probe and enzyme auxiliary signal amplification, which has the merits of simplicity, rapid response, low cost and high reliability.
In order to obtain more clear evidence, we examined the ultrastructure of control and i-Extract treated MCF7 cells at single-cell level.
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