The phrase "at sequential steps" is correct and usable in written English.
It can be used when describing a process or procedure that occurs in a specific order or series of stages.
Example: "The experiment was conducted at sequential steps to ensure accurate results."
Alternatives: "in successive stages" or "through a series of steps".
At the level of single cells, bacterial uptake, phagosomal maturation and degradation occur within a few minutes, and can be followed as sequential steps by imaging techniques.
In the Discussion, the 4-step model is laid out as sequential steps in a process.
Incorporating these results into known experimental evidence, we theorize the requirement of at least three sequential steps for native cellulose I crystallization: (1) formation of mini-sheets by van der Waals forces, (2) association of these sheets by hydrogen bonding into mini-crystals, and (3) the convergence of mini-crystals to form the crystalline microfibril.
Current DNA-based Gram identification methods include Gram stain-specific PCR [ 22], nested PCR [ 23], and PCR probe hybridization [ 24- 26], but all of these methods contain at least two sequential steps, and therefore they require a longer turnaround time to get a final result.
If the regulation is found at any of these sequential steps, stop and consider that version the most recent.
Staining was conducted at 4°C in several sequential steps in FACS staining buffer (1X PBS with 2% FCS, 0.1% NaN3), washing twice between steps.
TFIIE is an essential component of the RNA polymerase II transcription machinery [ 44], playing important roles at two distinct but sequential steps in transcription: pre-initiation complex formation-activation (open complex formation) and the transition from initiation to elongation [ 45].
The PCR program consisted of activation at 95°C for 15 minutes followed by cycling with sequential steps of denaturation at 95°C for 15 seconds, annealing at 58°C for 30 seconds and extension at 72°C for 15 seconds with the last step being used for data acquisition.
As the number of genes grows this becomes computationally expensive, however the problem is circumvented, as each gene can be added at a sequential step, through a process of merging and expanding the matrix.
The basic idea of the pipeline skeleton is to split the processing of an application into a series of sequential steps, with storage at the end of each step (Figure 7).
