Cells were harvested at sequential time points after chemotherapy, mixed with low-melting-point agarose, and spread on comet slides using a Trevigen CometAssay® Kit (Trevigen Inc., Gaithersburg, MD, USA).
In longitudinal studies, three-dimensional (3D) bone images are acquired at sequential time points essentially resulting in four-dimensional (4D) data for an individual.
Samples were fixed for microscopy (see below) at sequential time points including gametes (t = 0 h) and larvae at subsequent 24 h intervals until settlement.
By imaging at sequential time points and calculating the rate of change in object size, colonies with growth characteristics consistent with an M. tuberculosis colony can be identified.
Using such an approach, different animals have to be killed at sequential time points to determine the average course of tumour development and growth.
To compare the activities in the same neuronal population in response to two events at sequential time points, we used TetTag bi-transgenic mice in which neuronal activities at a given time window can be persistently labeled.
To determine whether the size of dark, PAS-positive granules changed with time, we arbitrarily divided dark granules into 3 category sizes and calculated the proportion of each size at sequential time points.
We argue that much will be learned through the acquisition of multiple tumour samples from different regions of the primary and metastatic tumour sites and at sequential time points in the disease course, including before, during therapy and at disease progression, to study the changing nature of tumours over time.
During the 2009 influenza pandemic we had access to a unique set of serum and lymphocyte samples, taken at sequential time points post vaccination, from vaccinees who elicited rapid and high protective antibody titers in response to the AS03 adjuvanted pdmH1N1 vaccine.
