Sentence examples for at second passage from inspiring English sources
The allogenic chondrocytes at second passage were transplanted with different scaffolds to repair rabbit cartilage injury.
Substrates were co-cultured with EPCs at second passage in 24-well culture plates.
At second passage, the incubation period for FSE appeared slightly longer, but this was not statistically different from the mean incubation period of the first passage experiment.
At second passage, the mean incubation period for the BSE agent appeared to be shortened but, unexpectedly, the incubation period for the FSE agent appeared to be longer compared to first passage data; this was not statistically significant, however.
At second passage, when the species effect of the BSE source is supposed to be erased, the lesion profiles of the FSE and BSE experiments also appeared very similar (Fig. 2A).
The mice were inoculated by both intra-cerebral and intra-peritoneal routes, from a bovine thalamus sample at first passage and from pools of brains from C57Bl/6 or SJL mice at second passage.
At first passage, the mean incubation periods were not statistically different between cheetah FSE and classical cattle BSE.
On this second passage in C57Bl/6 mice using 1% brain homogenates, similar molecular features to those described at first passage (H-type) were observed in mice (26/26) surviving after 469 d.p.i.
On second passage, most of the inoculated mice showed molecular features of the abnormal prion protein (PrPd) and brain lesions similar to those observed at first passage, but clearly distinct from those of classical BSE in this mouse model.
The reason for this tendency is unclear but it had already been reported for ovine BSE transmitted to this model (296 d.p.i at first passage to 365 d.p.i at 2nd passage) [17].
The explanation of shortened survivals of mice at this second passage is unclear, but it does not seem to reflect an adaptation of the H-type BSE agent in C57Bl/6 mice since the survival of mice that showed H-type features at third passage was longer.
