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DArT detects primarily dominant markers, mostly resulting from single nucleotide polymorphisms at restriction sites [6] at hundreds to thousands of arbitrary genomic loci [6], [7].
To generate the AAV-LS2L-RFP construct, dsREDexpress (Clontech) was subcloned into the LS2L shuttle plasmid at restriction sites EcoRV and HindII.
This method detects mutations at restriction enzyme sites in human genes.
The amplified NTRK1 gene was digested by EcoRI and XhoI at restriction sites introduced by the primers.
The pC-DEPC plasmid was linearized at restriction enzyme sites within the MCS, lane 1 in Figure 3B.
GFP PTEN was subcloned from pEGFP C2 PTEN (Leslie et al, 2000) into pQCXIP at restriction sites of AgeI- EcoRI.
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United States commanders chafe at restrictions the Saudis have placed on the American-led war on terrorism.
Political points were usually made obliquely, not through direct protest but by laughing at restrictions.
China had been invited to join the trade group but balked at restrictions that the deal would have placed on its financial sector and other areas.
Although Chinese officials vilify the Dalai Lama, the spiritual leader of the Tibetans, many Tibetans revere him and chafe at restrictions on religion imposed by China's Han rulers.
But he said he has examined the text of the agreement and closely looked at restrictions on Iran in the first 10 years, nuclear restrictions after 10 years, and non-nuclear components and consequences of a deal.
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