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Data are presented as mean±s.e., *P<0.05, **P<0.01 and ***P<0.001 for differences between diet groups at respective time points based on estimated marginal means.
Though the optical density values of formazan solutions from the SF membrane group were lower than those from the control group at respective time points (Fig. 4), these differences were not statistically significant (P > 0.05).
The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05).
The median of ratios of individual microarrays was adjusted to be the logarithm of the estimated DNA content at respective time points from equation (2).
To determine DNA copy number increase at various loci, individual microarrays were normalized based on the DNA content at respective time points as described elsewhere [6].
Briefly, treated cells were trypsinized and resuspended in the medium at respective time points.
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Animals (n = 5 7 at respective time point) were sacrificed by decapitation at 0.5, 1, 2, 3, 4, 5, 6, 8, 12, 18, and 24 h following i.p. injection.
The degradation of the scaffold was calculated from the following formula: mathrm{Degradation}%=left({W}_0-{W}_{mathrm{t}}right)/{W}_0times 100 (2)where W0 is the initial weight of the scaffold, and Wt is the weight of the scaffold at respective time point.
Comparisons between groups were made assuming parametric data using Student's t test for pairwise comparisons or one-way ANOVA with post hoc Tukey's test for multiple group comparisons at respective time point.
Native brains and spinals cord were prepared at the respective time points and snap-frozen at −70°C.
The size of tumors formed at the inoculation site was measured with standard procedure at the respective time points.
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