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In some experiments, hGDNF (Promega) or mature form of recombinant hGDF11 (R&D Systems) were added at respective concentrations of 100 and 50 ng/ml, similar to that used previously for GDNF (12) or suggested by pilot experiments for GDF11.
One example is frog muscle, where phosphocreatine is the most abundant metabolite (∼50 mM), followed by carnosine, total free amino acids, creatine and total ATP at respective concentrations of 19.5 mM, 11.7 mM, 11.0 mM, and 6.2 mM.
Each strain was cultured in Yeast Peptone Dextrose broth under four different growth environments; (i) in the absence and presence of B. javanica extract at respective concentrations of (ii) 1 mg/ml (iii) 3 mg/ml and (iv) 6 mg/ml.
Similar(57)
Granulocyte monocyte colony-stimulating factor (Leukine, Immunex, Seattle, WA, USA), soluble CD40 ligand (Immunex), and IL-7 (Immunex or R&D System, Minneapolis, MN, USA) were used at the respective concentrations of 100 ng/ml, 200 ng/ml and 10 IU/ml.
Radical scavenging activity of all test samples was plotted as % of ascorbic acid at respective concentrations.
The data were normalized to positive control (10 μM TO901317, 100% inhibition) and negative control (DMSO, 0% inhibition) values, with eq 1 used to derive the percent inhibition of individual chemicals at respective concentrations.
The activity of many extracts was comparatively better than the chelating capacity of PG (propyl gallate) at respective concentrations.
C = C_{o} e^{ - kt} (1 where Co and C are respective concentrations of the reactant at time, t = 0, and time, t = 2.0 s, while k is the pseudo-unimolecular rate constant in the Arrhenius expression (cf. Eq. 2).
The composition (respective concentrations) of the different substrates at the matrix and cytosolic sides of the carrier determine whether adenine nucleotides preferentially become exported or imported [ 11, 15].
Equations 10 and 11 give the respective concentrations of the intermediate I and the product at any time t.
Control samples contained the respective concentrations of DMSO.
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