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However, by D21 we could detect Gr1+ neutrophils, albeit at reduced frequency when compared to isotype-control treated mice (Figure 6b).
Cot-1 holes, regions of depleted RNA hybridization with a Cot-1 probe, were observed upon Xist expression; however, these were at reduced frequency and intensity in these somatic cells.
We note that iPSC colonies can be obtained without Lif, although at reduced frequency.
These survivors do not appear to have acquired linked genetic suppressors of lethality because they continue to transmit the R11 insertion to homozygous progeny at reduced frequency.
This is consistent with a model in which pervasive transcription is largely driven by the ability of RNA polymerases, given access, to initiate transcription from sub-optimal sites albeit at reduced frequency.
These benefits, however, do not seem to be mediated through reductions in the overall duration of injecting and might be associated with prolonged duration, albeit at reduced frequency during treatment.
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Thus, homozygotes survive at reduced frequencies, as scored at 7 to 10 days.
We analyzed daily and 2-week dosing frequencies to compare changes in treatment dynamics at reduced frequencies.
In contrast, hybrid F2[ g] (23%, N=48) and F2[ v] (59%, N=48) males performed courtship displays at reduced frequencies.
F2 vitripennis-giraulti hybrid males performed both early and late stages of courtship at reduced frequencies compared to parental vitripennis and giraulti males.
Although many species have undergone catastrophic declines and/or extinctions, others appear to be unaffected or persist at reduced frequencies after Bd outbreaks.
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