Matrix deposition of HA, however, was found to be higher than controls at PPS over the concentration range of 1.0 to 20 μg/ml but was only significant at 0.1 and 0.5 μg/ml PPS (P < 0.001) (Table 2).
Significant MPC proliferation was evident by day 3 at PPS concentrations of 1 to 5 μg/ml (P < 0.01).
For example, a patient admitted with PPS 10%, may be at PPS 40% after treatment, then drop back to 10% within a few days.
PPS increased the affinity between full-length ADAMTS-5 and TIMP-3, such that at PPS concentrations above 10 nM, 0.5 nM full-length ADAMTS-5 (ADAMTS5-1) was completely inhibited by 0.5 nM TIMP-3.
In the 5-day cultures, lower but significant (P < 0.001 to 0.05) stimulation of collagen type II synthesis by MPCs was still evident at PPS concentrations up to 20 μg/ml.
Because the 22 cases were randomly allocated to either Group 1 or Group 2 (each with 11 cases) when the study was first set up, Group 1 ended up with no representation at PPS 20% and PPS 80% and Group 2 with no PPS 40%, 70 % and 100.
