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At passage 0 more than 50% of the population was positive for Col I and ALP, but at passage 2, the proportion of cells expressing ALP increased.
The DREAM Act's shot at passage is slim.
One stopped growing early, at passage four.
MEFs were utilized at passage 1 2.
All experiments used cells at passage 8 14.
Three early lines stopped growing at passage two, one at passage nine, and one early line at passage 15.
The fraction of SSEA1 positive cells declined from 54% at passage 4 to 38% at passage 11 and 41% at passage 17.
NPCs at passage number ≤15 were used in all experiments.
Cells were used at passage numbers less than 30.
TTFs at Passage 3 were used to make iPS cells.
One promising outgrowth stopped growing at passage four.
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