The rate of the protein denaturation reaction at particular temperature can be generally described by the formula: <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0013249.e012.PNG" class= inline-graphic"/> where C is the concentration of undenatured protein at time t, n is the reaction order, and k is the reaction rate constant at this temperature [123].
Membrane fluidity has long been known to affect ability of strains to grow at particular temperature ranges.
Suggesting that, in order for the enzyme to perform the catalytic activity optimally at particular temperature, the enzyme should be in good conformation whereby the degree of quality of the enzyme may be judged based on the scores obtained from the Ramachandran plot.
The value of this ratio will indicate the type of conductive mechanism at particular temperatures.
Also, at particular temperatures, bridging between the surfaces of the crack wake by the inter-granular glass phase results in increased strength and fatigue retardation.
The percent retention of water (Wr) and weight-swelling ratio were determined by (4) W u = W t − W g W e × 100, where, W u is the solvent uptake capacity, W t the weight at regular time interval, W g the weight of dry cryogel and W e the weight of water in swollen gels at swelling equilibrium at a particular temperature.
In principle, heat-treating already takes place when steel is hot-rolled at a particular temperature and cooled afterward at a certain rate, but there are also many heat-treating process facilities specifically designed to produce particular microstructures and properties.
The protocol consists of an initial denaturation at 95°C for 4 minutes, followed by 40 cycles of denaturation at 95°C for 30 seconds, annealing at a particular temperature for each exon for 30 seconds as shown in Additional file 1, and extension at 72°C for 30 seconds, followed by a final extension at 72°C for 7 minutes.
The values for Tm, Δ G°T, and Δ Hm were obtained by fitting the data to a two-state model, where Δ G°T is a change in the free energy at a particular temperature and Δ Hm is the enthalpy at the melting temperature, Tm.
Insects placed on a surface that provides a temperature gradient (warmer at one end and cooler at the other) often congregate in a narrow band at a particular temperature, providing behavioral evidence of sensitive thermoreception.
By varying the composition of a petroleum mixture, it is possible to attain a desired viscosity at a particular temperature.
