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During operation up to 26.6 kPa, O2 partial pressures and pH in the cell-culture medium do not change compared to control cultures kept at normal atmosphere.
The solid was filtered and dried at normal atmosphere.
Plates were incubated for 5 days at 37°C at normal atmosphere.
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The plates were incubated at 37°C for 5 days at a normal atmosphere.
After incubation of the desalted enzyme at 30°C under normal atmosphere and without inhibitor for two hours, more than 20% of the initial activity was lost, while no loss of activity was observed when stored under argon or with the inhibitor 4-HBA, respectively.
The pH was adjusted to 7.4 and the cells were incubated at 37°C in normal atmosphere supplemented with 5percentnt carbondioxide.
Each micro plate was then sealed with parafilm and incubated for 5 7 days at 37°C in normal atmosphere.
Vertebrate cells were incubated at 37°C in a 5% CO2 atmosphere and invertebrate cells were incubated at 27°C in normal atmosphere.
For the controls and for the samples, two plates of each medium were used; one plate was maintained at 37°C in a normal atmosphere and another plate was maintained at 37°C with 10% CO2 and high humidity.
The mixed solution was stirred with a magnetic stirrer at room temperature for 15 min, and was thermally decomposed in an oven under normal atmosphere at different temperatures of 600, 700, 800, and 900 °C for 6 h and left to cool down to room temperature before being ground to obtain LSMO nanoparticles.
After annealing in normal atmosphere at 600 °C for 2 h, TiO2 crystallized and consequently, nanotubular TiO2 arrays containing anatase and rutile phases formed.
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