At least three functioning town-house garages remain in New York, including those of Lewis Morris and the banker George F. Baker, who had Delano & Aldrich design a garage wing for his Georgian-style house at Park and 93rd.
Cells that had at least one functioning copy of each BRCA grew normally when mixed with the PARP inhibitor, suggesting healthy cells tolerate the chemical well.
Since every SMA patient has at least one functioning SMN2 gene, it has become a target for therapeutic interventions, and most pre-clinical studies have focused on up-regulating SMN levels by some means [14], [15], [16], [17], [18], [19], [20], [21], [22], [23], [24].
The Toronto festival has always served at least two functions -- a place where distributors can unveil releases before an appreciative audience (as well as the largest annual concentration of North American entertainment journalists) and a market where producers and directors can try to interest distributors in buying the rights to their films.
The existence of each tier in the regulatory hierarchy is justified by the assignment of a unique task and each invariably performs at least two functions: to activate the regulators in the next tier and to perform one other activity distinct from that of the next tier.
For enzyme coupling, construction of fusion protein has been developed as an effective method for cofactor regeneration, where two coding genes are combined by a short linker sequence to yield a single polypeptide exhibiting at least two functions (Calam et al. 2016; Farrow et al. 2015; Suehrer et al. 2014).
It is likely that the ancestral roles of the Kinesin-13 family may have included at least two functions at disparate cellular locations: one on the mitotic spindle and a second at the tip of the flagellum, with the uniting feature being the depolymerising action of these proteins.
