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At its maximum effect, a second set of hemodynamic and Doppler measurements was performed (Additional file 1: Figure S1).
Once the predator reaches the prey, the defence will be at its maximum effect.
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SN-38 induced an arrest at S/G2/M phase, reaching its maximum effect at 12 hours.
The analysis of concentration-response curves indicates that the potency (nM) of AT-II contraction at 20, 50 and 80% of its maximum effect did not change significantly (P > 0.05) among the experimental groups (Table 3), whereas the contractile efficacy of AT-II (intrinsic activity) was drastically increased in D and to a less extent in DL.
In fact, pharmacological evidence indicated that in aortas from all the groups of rats: 1) AT-II maintained a similar potency at 20, 50 and 80% of its maximum effect in determining contracture; 2) irbesartan, as well as irbesartan plus PD123319, reduced analogously AT-II contracture.
Notwithstanding this, AT-II potency (nM) at 20, 50 and 80% of its maximum effect was not significantly different from normo (N and NL) to hyperglycaemic (D and DL) aortas (Table 3).
The concentration at which NK-4 exerted its maximum effect differed when assessed by growth or neurite-outgrowth: a maximal effect was obtained at 250 nM as assessed by cell growth, whereas a higher dose of up to 2.5 µM was needed for neurite-outgrowth.
The longer exposure of lower dose (60 uM) of ST showed time-dependent effect for apoptosis; however, the higher dose (90 uM) achieved its maximum effect at 48 h after the treatment.
In spontaneously contracting rabbit jejunum preparations, VBME caused a concentration-dependent stimulant effect at 0.01-0.3 mg/mL, reaching to its maximum effect of 67.33 ± 2.67% (mean ± S.E.M; n = 5) of ACh maximum.
On the other hand, the scalp distribution of the figure effect within the N1 time-window was not significantly different from the scalp distribution of the figure effect at its maximum (between 200 and 260 ms).
The effects of chlorpyrifos at its maximum tolerated dose of 1 mg/kg can thus serve as a benchmark for parallel comparisons of the effects of diazinon and parathion as evaluated in the present study.
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