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At injection, background NIR fluorescence is observed only in the intestinal area due to autofluorescence of chlorophyll in the mouse chow.
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Transgenic mice were generated by pro-nucleus injection (FVB/N background).
For peptides not clearly identified at all time points, a retention time window was used identical to that in other injections with background signal integrated and used for analysis.
Prior to probe injection, emission was at a background level due to autofluorescence.
The spray atomization enhanced by increasing injection pressures is more pronounced at elevated background pressures.
However, the mercury counts are at the normal background level again prior to injection of the next sample.
CPK levels in sham-treated animals remained at background levels, indicating that intramuscular injection per se is not responsible for enzyme elevation).
High background radioactivity from tracer remaining at the injection site necessitated segmental excision of the primary lesion before the sentinel node could be identified with the probe in 53 patients (34%).
Point this at the background panel.
Before and during the PET study, nine arterialized venous blood samplings were obtained: at background, 30 sec, and 1, 3, 5, 10, 20, 30 and 50 min after the tracer injection from the antecubital vein contralateral to the intravenous FBPA injection side, heated in a heating blanket.
60 Surgical instruments and pathology slides appear to stay at background radiation levels, while measurable contamination of absorptive surgical sponges and other materials used in proximity to the injection site is observed.
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