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This was important to confirm since the two sample tests were done at individual spot level.
Thus, at least at the scale of the axes in which different spots are well separated, we found no indication of MUs with distinct response properties in individual spots.
This is not an artifact of spot clustering in the mutants, since individual spots are resolvable from aggregations of spots.
We assessed the changes for individual spots, as well as for all spots in total.
PALO ALTO — UC Irvine freshman Luciane Lee shot an even-par 71 on the second round of the NCAA West Regional at the Stanford Golf Course to move into contention for one of two individual spots at the NCAA Championships.
The ScanArray Express HT microarray scanner (Perkin Elmer) was used to scan the signal of the individual spots at 543 nm (Cy3) and 633 nm (Cy5).
The transformation of the distribution of the 201 spots also worked reasonably well at the level of individual spot intensities.
Hybridized chips were scanned with a GenePix 4000B Axon Scanner, using GenePix Pro 6.1 software (Molecular Devices, Sunnyvale, CA) at 5 μm for individual spot evaluation.
Two out of the 49 discriminating genes (HLA-DRA and STAT1) were selected by two individual spots located at different places on the array, emphasizing the reproducibility of the gene signature identified.
The minimal distance between two individual spots should therefore be at least 1.2 mm (2 × 600 μm).
Non-crossover events are thought to have different distributions across the genome than crossovers; Holloway et al. (2006) found this in sperm-typing studies at individual hot spots.
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