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Briefly, 5 × 105 cells were harvested and washed twice (180 g, 10 min, 4°C) with PBS at indicated points in time.
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2.5 3 mL cell cultures was collected from indicated cultures at indicated time points.
Recipients were sacrificed at indicated time points and haematological organs were analysed in detail.
Cells were harvested and counted at indicated time points.
Apoptosis was analyzed at indicated time points via the Annexin V assay.
Livers were harvested at indicated time points (see below) under anesthesia and analgesia as described above.
Cells were collected or fixed at indicated time points and subjected to specific analyses.
To determine expression levels of the genes, iWAT was acutely dissected from the mice at indicated time points after treatment.
Embryos were isolated at indicated time points.
Cells were harvested at indicated time points for specific experiments.
Adherent and suspension cells were collected at indicated time points.
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