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Within the Cct subfamily, it is surprising that subunits of a heteromeric octamer are not uniformly expressed at identical levels (Figure 8).
However, we found that different genes co-amplified on the same amplicon are not necessarily over-expressed at identical levels (Figures 1B, 4A and B).
Treatment of immunoprecipitates containing these CTFs with λPPase resulted in the appearance of three discrete bands corresponding to dephospho-forms of C99, C89, and C83, all of which were present at identical levels in both wild-type and Thr668Ala knock-in mice.
As expected for single-copy insertions, quantitative PCR showed that the mRNA for both pgp-3 wt and pgp-3 ΔF508 was expressed at identical levels (Fig. 1B).
By quantifying two Tsa1p-specific tryptic peptides (GLFIIDPK and HITINDLPVGR) and nor-malization of their peak areas to peptides specific for Triosephosphate isomerase (ASGAFTGENSVDQIK and KPQVTVGAQNAYLK), we found that Tsa1p is expressed at identical levels in K6001 and K6001-B7.
Video microscopy was performed with an Axioplan (Zeiss) upright microscope and 544 frames were digitally captured at identical levels of magnification (10×) and frame rate (250 frames per second).
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HPRT, which was expressed at nearly identical levels in both libraries, was used for normalization.
Additionally, unlike the case for HER2, our primary comparison shows optical and digital reads are at almost identical levels, independent of the format (whole slides versus TMA).
In C7/10 cells, miR-1889-3p and miR-1889-5p wexpressedssed at nearly identical levels, suggesting that both strands of the miRNA:miRNA* duplex are loaded equally into RISC as mature miRNAs.
Importantly, neither CD8+ nor CD4+ T cell recruitment was altered by TIMP-1 deficiency, as both populations peaked at almost identical levels between days 7 10 p.i. as in WT mice.
The proposed VSC DTC maintains enhanced transient performance similar to the LUT DPC and keeps the steady-state harmonic spectra at the identical level as the VC strategy when the network is strictly balanced.
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