"We intend to look at the further steps we need to take towards a full economic union to complete our monetary union," he said Wednesday.
Incubation with poly-GR and poly-GP antibodies was done overnight at 4 °C, further steps were an incubation with a rabbit anti-rat antibody (1 2000) for 1 h at room temperature, and a final processing on the Ventana BenchMark XT using the UltraView Universal DAB Detection Kit (Roche).
Note that this premise merely assumes the abstraction of the concept of some sort of unity, found, for example, in the experience of single bodies (which really is common experience), and not the formation of the concept of absolute unity, which would require (at least) the further steps indicated below.
After one day of fixation at 4°C, the samples were washed with 5% glucose in 0.1 M cacodylate buffer which was exchanged every 10 days and stored at 4°C until further steps.
After removal of cell debris by centrifugation (30 min with 6,000 g at 4°C), all further steps were performed according to the IMPACT-CN protocol (New England Biolabs, Frankfurt a. Main, Germany) with the following changes: For washing, phosphate buffer with 1,000 mM NaCl was used to remove all non-specifically bound proteins.
Ministers will then consider further steps at another meeting of the education council later this year.
Russia and China, which have opposed economic sanctions, are likely to resist further steps at the United Nations, while the Americans and Europeans have warned of imposing more on their own.
In a system that requires unanimity, grumbling from Finland and the Netherlands is a reminder that even if the limited deal at the summit holds, further steps will be hard.
An important potential obstacle was removed in November when Olli Rehn, the EU's economic commissioner, accepted that Spain was making sufficient fiscal effort and needed to take no further steps at present.
