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This study has revealed the importance of serially assessing pyruvate metabolism under in vivo conditions, at frequent time points as heart failure progresses.
Further, by using hyperpolarized C MRI to measure [1-C]pyruvate oxidation by the PDC serially at frequent time points, we identified a temporal association between reduced pyruvate oxidation and the onset of overt heart failure.
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Unfortunately, technical and other considerations make assaying at more frequent time points impractical.
A GWG rate of 0.22 kg per week has previously been estimated during the first trimester [ 28] and the most frequent time point for the initial visit to the antenatal clinic is at ten weeks of gestation.
Standard methods for monitoring stream and groundwater conditions typically measure at a small set of geographic points (e.g., stream flow at gages, groundwater elevation at wells) at frequent time intervals.
The participants were fed with standardized light meals at frequent set time points throughout the experiment to maintain blood glucose and digestion as constant as possible.
For the SF-6D dimensions physical functioning and role limitation, the worst category was most frequent at both time points, but improved from level 4 to level 3 for the dimensions social functioning and mental health from admission to discharge.
In some animals foamy macrophages were frequent at the later time points.
However, FDA is easier to apply in situations with more frequent sampling, sampling at unequal time points and missing data.
Terminal ramifications progressively spread at longer time points (Figure 2A), being always more frequent and extended in postnatal neurons, whose arbors eventually became 4.7 times larger than their embryonic counterparts (Figure 2C).
Limitation: We only measured at one time point of one month and did not evaluate changes at other time points Rotator cuff tears are among the most frequent and debilitating upper extremity injuries, with over 250 000 surgical repairs performed each year in the United States.
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