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After each blood sample was collected, each fish was euthanized by cranial impact and its second and third gill arches quickly excised to a 1.5 mL cryovial and frozen in liquid nitrogen at for future analysis.
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One swab was tested locally; the second was stored at −20°C for future quality control testing at the ICRL.
Blood samples were collected at this time and stored at −80°C for future analysis.
The voucher number of the plant is HKNU 201203 deposited at the herbarium of Plant Extract Bank at Hankyong National University for future reference.
Both tibiotarsi and femora were left intact at the stifle joint and stored at −20 °C for future measurement.
At different time points, material is stored at −80°C for future analyses (Table 1).
The solvent was removed, filtered, and dried at room temperature and residues were stored at −20°C for future use.
The aqueous layer was lyophilized (at -45°C) and the dry powder was stored at -20°C for future use.
These samples were stored at 4°C if used within a few days, or at −80°C for future use.
The supernatant was collected after centrifugation at 15000 × g for 5 min and stored at −20°C for future use.
The residue was kept at −20 °C for future use.
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