Sentence examples for at first passage from inspiring English sources
At first passage, the mean incubation periods were not statistically different between cheetah FSE and classical cattle BSE.
On this second passage in C57Bl/6 mice using 1% brain homogenates, similar molecular features to those described at first passage (H-type) were observed in mice (26/26) surviving after 469 d.p.i.
The reason for this tendency is unclear but it had already been reported for ovine BSE transmitted to this model (296 d.p.i at first passage to 365 d.p.i at 2nd passage) [17].
After 24 h the media was changed non-adherent cells were discarded and the adherent decidualized stromal cells (DSCs) were allowed to attain confluence and used for experimentation at first passage.
PrPres was extracted and detected as previously described [15], from mice infected at first passage performed by inoculation of 10% brain homogenates with the different TSE sources from ruminants.
One subgroup showed the histopathological features seen in H-BSE at first passage, and especially a low amount of PrPd which was exclusively detected, by Congo red staining, as amyloid plaques (Figure 3A1, A2, C, E1, E2).
Due to elimination of damaged cells, populations of cultured cells seem to become "younger", at least at first passages.
The allogenic chondrocytes at second passage were transplanted with different scaffolds to repair rabbit cartilage injury.
At second passage, the incubation period for FSE appeared slightly longer, but this was not statistically different from the mean incubation period of the first passage experiment.
At second passage, the mean incubation period for the BSE agent appeared to be shortened but, unexpectedly, the incubation period for the FSE agent appeared to be longer compared to first passage data; this was not statistically significant, however.
