The value of each EGV at every sampled location was extracted and saved in a separate layer.
Thus, the model is able to determine the origin of larvae ending up at each sampling location, identifying potential source populations.
It is essential to establish an adequate sampling protocol that can be applied rigorously at each sampling location and time.
Overall microbiomes (rarefied to 24,509 reads for each sample) as well as the community of locally stable microbial associates (OTUs present in 100% of the samples at each sampling location; rarefied to 23,940 reads for each sample) and the core microbiome (OTUs present in all samples; rarefied to 23,890 reads for each sample) were determined.
The trajectories and the associated climatic conditions of air masses arriving at the sampling location on the day of the precipitation event were determined with the HYSPLIT model version 4 (HYbrid Single-Particle Lagrangian Integrated Trajectory, http://ready.arl.noaa.gov/ready/HYSPLIT4.html).arl.noaa.gov/ready/HYSPLIT4.html
For Coptotermes cf. acinaciformis, microscopy was carried out at the sampling location using a Swift Field Master phase contrast field microscope (Swift Optical Instruments, Schertz, TX) and imaging using a Canon G9 camera (Canon, Tokyo, Japan).
Undisturbed samples were collected at fixed depths and analysed for BD and SOC content at each sampling location and continuous soil profiles were reconstructed using equal-area splines.
At each sampling location, samples were collected into polyethylene bottles in triplicate.
Percentage cover of corals, sponges, macroalgae and coralline algae was estimated to the nearest 1% and the dominant scleractinian and macroalgal genera were recorded at each sampling location.
At each sampling location, we collected bees during mornings and afternoons by walking haphazard trajectories and catching all visible workers, males, and queens with sweep nets.
