Exact(36)
In order to assess the ability of the system to promote ECM mineralization, MC3T3-E1 cells were plated at equal densities on each disk type and were grown in the presence of differentiation medium for 12 days.
The number of genes peaking in expression were plotted against time of day using GraphPad Prism 4. Canton-S strain of Drosophila melanogaster were raised on a cornmeal-molasses-yeast medium at equal densities to ensure uniform size.
HMEC-L were plated at equal densities, after 12 h incubated in the different experimental conditions and stained with Annexin V-FITC (Boehringer Manheim) and propidium iodide (PI) (Interchim).
Cells were seeded at equal densities in 6-well plates and, after reaching confluence, they were washed and incubated for one additional hour in the same medium (control) or medium supplemented with γ-irradiated LPS purified from E. coli serotype 055∶B5 (Sigma-Aldrich) or mouse recombinant tumor necrosis factor-α (TNF-α, 410-MT; R&D Systems).
Cultures were established by first growing morphotypes for 18 hours in microcosms (30 ml glass universals with loose plastic lids, containing 6ml of King's Medium B (KB) supplemented with 0.0024% pantothenic acid) at 28°C in a 200 rpm orbital shaker, an then inoculating into new microcosms at equal densities, with total mixture density 100-fold less than the maximum afforded by the microcosms.
Cells were harvested and seeded at equal densities in triplicate in 96-well plates.
Similar(24)
NB cells were plated at equal density on 10-cm dishes.
To compare the growth rate of wild type and mutant cells, mycelia were harvested and dispersed by grinding and inoculated into fresh medium at equal density.
HeLa cells were plated at equal density and transfected with either flag-tagged Atx1 constructs or with empty pCMV flag vectors.
We cultured flies at equal density in plastic vials with cotton plugs on 8 ml of standard Drosophila cornmeal and molasses medium at 23°C (except for heat stress treatment, below).
HeLa cells were plated at equal density and treated with 50 μM of indicated test compound.
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