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At each step of the protocol, we obtained images of the common carotid artery.
Blood samples were collected at each step of the protocol.
At each step of the protocol the following parameters were recorded: SAP, MAP, DAP, HR, breathing rate, CO, and pulse oximeter oxygen saturation.
It would be important if they provide information on how much material they recover at each step of the protocol (Post OH step, 3'end repair, ligation…) as well as the reaction volumes of all steps (Results).
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Each step of the experimental protocol should be performed methodically to obtain reliable results.
At each step of the study, patients and physicians will have to give their written consent to participate after being informed about the study protocol.
Additional analyses of patient attrition at each step in the protocol also were undertaken.
Additional analyses of patient attrition at each step in the protocol from screening through the 12 month assessment also will be undertaken.
At this step of the protocol dynamic Min patterns formed on top of the membrane.
The initial step of the protocol entails at least one large dilution step.
FACS analyses performed at the end of step 1 showed that the DE-enriched cells were homogeneously positive for CXCR4 and after the fourth step of the protocol (day 12), 80% of cells expressed PDX1 (Fig. 2n,o).
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