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These results indicate that sows can exhibit clear preferences for enrichment type, with the sows interacting with the rope significantly more often throughout the study, at each sampling hour.
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Tracheal samples were obtained from all intubated patients at each sampling interval (baseline and every 48 hours) using a sterile suction catheter placed into the endotracheal tube.
To study the oscillatory patterns of these floral regulators in 45OX-5 tricegenic rice, leaf RNA samples were analyzed at each 4-hour interval under 14 h light/10 h dark cycle condition.
After incubation, 500 pg LPS was added to each sample for 4 hours at 37°C.
After incubation, 500 pg LPS was added in each sample for 4 hours at 37°C.
Due to inadequate volume, one sample at 1 hour, 2 samples each at 2 and 4 hour time-points could not be assayed.> -wrap-foot> interquartilerange range, SD: standard deviation, AFB: acid fast bacillus, MGIT: Mycobacterial growth indicator tube, BCG: Bacillus Calmette Guerin.
DNA was extracted using a QIAcube and QIAamp DNA mini kit tissue protocol, with the addition of heating each sample at 90°C for 1 hour after incubation of the sample at 56°C with 180 μL of Buffer ATL plus 20 μL Proteinase K.
Aliquots of each sample were hybridized (16 hours at 45°C) to an array (GeneChip® Rat Expression Set 230A arrays; Affymetrix).
A centrifugation from the homogenized solution was done to each sample at 3500 rpm for half an hour.
Among non-ICU patients with blood cultures that were negative at 48 hours, sampling at all centers was fixed at 10%.
Participants were advised to avoid physical activity for 48 hours and to fast for at least 10 hours prior to each sample collection (i.e. baseline and 12 weeks).
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