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This is thought to be due to genetic drift acting on already small populations that go through population bottlenecks at each host generation [9].
At each host generation, a random number of individuals of each genotype is added to or removed from the populations, the maximum change being a fraction F (here, F = 0.05).
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In the discrete-time models (the standard model and the sequential-updating model), the following processes occur at each timestep: host reproduction, host selection, host mutation, pathogen reproduction, pathogen selection, and pathogen mutation.
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First, we conducted a Petri dish inoculation bioassay with both genotypes, SA 27774 (early resistance) and SA 4087 (late resistance) [ 31] in order to define the infection process stages at which each host prevented parasitism and to obtain a precise description of the defense response timing (additional file 1, Table 1).
In each host at both temperatures and at both time points, the consequences of the mutations in the promoter were the same.
For most simulated conditions, evolution to high virulence in the more competent Host 1 was little dependent on inoculum flow from Host 2, while in Host 2, it depended on transmission from Host 1. Virulence evolution bifurcated in each host at low, but not at high, vector densities.
Many key properties of this symbiosis still remain to be characterized, including the exchange of metabolites and signals between the symbiont and the host and the mechanism of symbiont acquisition at each new host generation (i.e., symbiont transmission mode).
Fitness was measured for each strain with each host at each temperature, and the fitnesses of the mutant strains were compared to that of the ancestor.
MLVA discriminated among the otherwise indistinguishable isolates from different animals and from isolates collected at different time points within each host, with different VNTR alleles being detected at multiple dates and tissue sites.
Institutional review was performed at each study host site.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com