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For statistical analyses, data from all effector:target ratios at each concentrations were pooled together.
Figure 5A shows that the purified (His 6-p53 protein antigen at eacHis 6-p53rations was able to clearly differentiate proteinoantigeny positive seratfrom thoseachat were negative.
For the multi-cell Flexstation experiments, the averaged percent inhibitions of fluorescence at each concentrations (n=3) were used to construct the concentration response curves and to estimate the IC50 values.
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The inset shows individual spectra at each concentration.
Six replicate measurements were recorded at each concentration level.
Calibration curves were established with three replicates at each concentration.
At each concentration of denaturant, HX measurements are performed over a range of pH values.
Reproducibility was evaluated by measurement of the sample five times at each concentration.
The experiments were repeated 3 times at each concentration of each compound.
The calibration curves for each biosensor at each concentration (0.015, 0.15, 1.5, and 8.2) were plotted.
The potentials obtained for the five analyses were averaged at each concentration.
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