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As fibre strength was seen to decrease after magnesium coating, the Weibull parameters (shape and scale) used to calculate IFSS were obtained using their real tensile strength data (i.e., from the coated fibre properties at each coating time).
Poly allylamine hydrochrloride)–AlexaFluor 594 (PAH-AF594) and bovine serum albumin AlexaFluor 488 (BSA-AF488) were complementarily coated, and the fluorescent intensity for each dye was determined at each coating layer.
The substrate was heat-treated at 60°C for 10 min at each coating.
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Thereafter, the films were dried after each coating at preheating temperature (approximately 250 C) in a hot air oven for 2 min. This process was repeated 15 times to obtain the required dense film of appropriate thickness.
Each coating antibody was diluted at 1 : 250 in PBS and incubated overnight at 4°C.
Wait at least 30 minutes between each coat.
Use light coats, and wait for each coat to at least partially dry before applying the next one.[20].[20]
As far as you're concerned, your coats are "at the coat check".
You should apply at least 2 to 3 coats and wait for each coat to dry before applying the new coat.
Metal electrodes at each end are coated with an alkaline earth oxide that gives off electrons easily.
From these 2D images, some measurements are carried out, giving an estimation of the diameter values for a sample of particles at each step of the coating process.
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